Composite

Part:BBa_K4759270:Design

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-08)


T7-RBS4-hemB-RBS2-hemD-RBS2-hemC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1068
    Illegal EcoRI site found at 1845
    Illegal EcoRI site found at 2136
    Illegal PstI site found at 553
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1068
    Illegal EcoRI site found at 1845
    Illegal EcoRI site found at 2136
    Illegal PstI site found at 553
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1068
    Illegal EcoRI site found at 1845
    Illegal EcoRI site found at 2136
    Illegal BamHI site found at 1062
    Illegal BamHI site found at 1839
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1068
    Illegal EcoRI site found at 1845
    Illegal EcoRI site found at 2136
    Illegal PstI site found at 553
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1068
    Illegal EcoRI site found at 1845
    Illegal EcoRI site found at 2136
    Illegal PstI site found at 553
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2748


Design Notes

Cloning the genes from E. coli genome and use RBS2 to link them to constructed the plasmid to express hemB 、hemD 、hemC under the same promoter.


Source

E. coli genome

References